cd25 elisa Search Results


cd25 elisa  (R&D Systems)
93
R&D Systems cd25 elisa
A Compared with the PTPN22 WT, PTPN22 ΔP1 can increase remarkably the phosphorylation levels of ZAP-70, Lck and Erk. B, C, D Western blot and qPCR assays were used to test the protein and mRNA expression levels of <t>CD25,</t> CD69 and IL-2, and the results shown that the protein and mRNA expression levels of CD25, CD69 and IL-2 increased significantly in PTPN22 ΔP1 transfected cells. Western blot assays were used to test the protein expression levels of CD25 and CD69 (B-C) or qPCR assays were used to test the mRNA expression levels of CD25, CD69 and IL-2 (D). E, F, G Deletion of P1 domain of PTPN22 will not affect its enzyme activity. pNPP hydrolysis with PTPN22 catalytic domain and PTPN22 ΔP1 (E) or Kinetics parameters for the wild-type and the mutants of PTPN22 toward pNPP (F,G). Data information: All Experiments were carried three times (A-E) with similar results. In (A-C), Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.
Cd25 Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd25 elisa - by Bioz Stars, 2026-04
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human sil 2r α cd25 elisa kit  (Multi Sciences (Lianke) Biotech Co Ltd)
91
Multi Sciences (Lianke) Biotech Co Ltd human sil 2r α cd25 elisa kit
A Compared with the PTPN22 WT, PTPN22 ΔP1 can increase remarkably the phosphorylation levels of ZAP-70, Lck and Erk. B, C, D Western blot and qPCR assays were used to test the protein and mRNA expression levels of <t>CD25,</t> CD69 and IL-2, and the results shown that the protein and mRNA expression levels of CD25, CD69 and IL-2 increased significantly in PTPN22 ΔP1 transfected cells. Western blot assays were used to test the protein expression levels of CD25 and CD69 (B-C) or qPCR assays were used to test the mRNA expression levels of CD25, CD69 and IL-2 (D). E, F, G Deletion of P1 domain of PTPN22 will not affect its enzyme activity. pNPP hydrolysis with PTPN22 catalytic domain and PTPN22 ΔP1 (E) or Kinetics parameters for the wild-type and the mutants of PTPN22 toward pNPP (F,G). Data information: All Experiments were carried three times (A-E) with similar results. In (A-C), Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.
Human Sil 2r α Cd25 Elisa Kit, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human sil 2r α cd25 elisa kit - by Bioz Stars, 2026-04
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human cd25 il 2rα quantikine elisa kit  (R&D Systems)
94
R&D Systems human cd25 il 2rα quantikine elisa kit
A Compared with the PTPN22 WT, PTPN22 ΔP1 can increase remarkably the phosphorylation levels of ZAP-70, Lck and Erk. B, C, D Western blot and qPCR assays were used to test the protein and mRNA expression levels of <t>CD25,</t> CD69 and IL-2, and the results shown that the protein and mRNA expression levels of CD25, CD69 and IL-2 increased significantly in PTPN22 ΔP1 transfected cells. Western blot assays were used to test the protein expression levels of CD25 and CD69 (B-C) or qPCR assays were used to test the mRNA expression levels of CD25, CD69 and IL-2 (D). E, F, G Deletion of P1 domain of PTPN22 will not affect its enzyme activity. pNPP hydrolysis with PTPN22 catalytic domain and PTPN22 ΔP1 (E) or Kinetics parameters for the wild-type and the mutants of PTPN22 toward pNPP (F,G). Data information: All Experiments were carried three times (A-E) with similar results. In (A-C), Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.
Human Cd25 Il 2rα Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cd25 il 2rα quantikine elisa kit - by Bioz Stars, 2026-04
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human cd25 il2 r alpha duoset elisa kit  (R&D Systems)
94
R&D Systems human cd25 il2 r alpha duoset elisa kit
A Compared with the PTPN22 WT, PTPN22 ΔP1 can increase remarkably the phosphorylation levels of ZAP-70, Lck and Erk. B, C, D Western blot and qPCR assays were used to test the protein and mRNA expression levels of <t>CD25,</t> CD69 and IL-2, and the results shown that the protein and mRNA expression levels of CD25, CD69 and IL-2 increased significantly in PTPN22 ΔP1 transfected cells. Western blot assays were used to test the protein expression levels of CD25 and CD69 (B-C) or qPCR assays were used to test the mRNA expression levels of CD25, CD69 and IL-2 (D). E, F, G Deletion of P1 domain of PTPN22 will not affect its enzyme activity. pNPP hydrolysis with PTPN22 catalytic domain and PTPN22 ΔP1 (E) or Kinetics parameters for the wild-type and the mutants of PTPN22 toward pNPP (F,G). Data information: All Experiments were carried three times (A-E) with similar results. In (A-C), Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.
Human Cd25 Il2 R Alpha Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cd25 il2 r alpha elisa kit  (R&D Systems)
93
R&D Systems human cd25 il2 r alpha elisa kit
A Compared with the PTPN22 WT, PTPN22 ΔP1 can increase remarkably the phosphorylation levels of ZAP-70, Lck and Erk. B, C, D Western blot and qPCR assays were used to test the protein and mRNA expression levels of <t>CD25,</t> CD69 and IL-2, and the results shown that the protein and mRNA expression levels of CD25, CD69 and IL-2 increased significantly in PTPN22 ΔP1 transfected cells. Western blot assays were used to test the protein expression levels of CD25 and CD69 (B-C) or qPCR assays were used to test the mRNA expression levels of CD25, CD69 and IL-2 (D). E, F, G Deletion of P1 domain of PTPN22 will not affect its enzyme activity. pNPP hydrolysis with PTPN22 catalytic domain and PTPN22 ΔP1 (E) or Kinetics parameters for the wild-type and the mutants of PTPN22 toward pNPP (F,G). Data information: All Experiments were carried three times (A-E) with similar results. In (A-C), Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.
Human Cd25 Il2 R Alpha Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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serum catalog numbers dr2a00 sr2a00 pdr2a00  (R&D Systems)
93
R&D Systems serum catalog numbers dr2a00 sr2a00 pdr2a00
A Compared with the PTPN22 WT, PTPN22 ΔP1 can increase remarkably the phosphorylation levels of ZAP-70, Lck and Erk. B, C, D Western blot and qPCR assays were used to test the protein and mRNA expression levels of <t>CD25,</t> CD69 and IL-2, and the results shown that the protein and mRNA expression levels of CD25, CD69 and IL-2 increased significantly in PTPN22 ΔP1 transfected cells. Western blot assays were used to test the protein expression levels of CD25 and CD69 (B-C) or qPCR assays were used to test the mRNA expression levels of CD25, CD69 and IL-2 (D). E, F, G Deletion of P1 domain of PTPN22 will not affect its enzyme activity. pNPP hydrolysis with PTPN22 catalytic domain and PTPN22 ΔP1 (E) or Kinetics parameters for the wild-type and the mutants of PTPN22 toward pNPP (F,G). Data information: All Experiments were carried three times (A-E) with similar results. In (A-C), Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.
Serum Catalog Numbers Dr2a00 Sr2a00 Pdr2a00, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kits  (R&D Systems)
92
R&D Systems elisa kits
A Compared with the PTPN22 WT, PTPN22 ΔP1 can increase remarkably the phosphorylation levels of ZAP-70, Lck and Erk. B, C, D Western blot and qPCR assays were used to test the protein and mRNA expression levels of <t>CD25,</t> CD69 and IL-2, and the results shown that the protein and mRNA expression levels of CD25, CD69 and IL-2 increased significantly in PTPN22 ΔP1 transfected cells. Western blot assays were used to test the protein expression levels of CD25 and CD69 (B-C) or qPCR assays were used to test the mRNA expression levels of CD25, CD69 and IL-2 (D). E, F, G Deletion of P1 domain of PTPN22 will not affect its enzyme activity. pNPP hydrolysis with PTPN22 catalytic domain and PTPN22 ΔP1 (E) or Kinetics parameters for the wild-type and the mutants of PTPN22 toward pNPP (F,G). Data information: All Experiments were carried three times (A-E) with similar results. In (A-C), Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.
Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kits - by Bioz Stars, 2026-04
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immunosorbent assay  (R&D Systems)
93
R&D Systems immunosorbent assay
A Compared with the PTPN22 WT, PTPN22 ΔP1 can increase remarkably the phosphorylation levels of ZAP-70, Lck and Erk. B, C, D Western blot and qPCR assays were used to test the protein and mRNA expression levels of <t>CD25,</t> CD69 and IL-2, and the results shown that the protein and mRNA expression levels of CD25, CD69 and IL-2 increased significantly in PTPN22 ΔP1 transfected cells. Western blot assays were used to test the protein expression levels of CD25 and CD69 (B-C) or qPCR assays were used to test the mRNA expression levels of CD25, CD69 and IL-2 (D). E, F, G Deletion of P1 domain of PTPN22 will not affect its enzyme activity. pNPP hydrolysis with PTPN22 catalytic domain and PTPN22 ΔP1 (E) or Kinetics parameters for the wild-type and the mutants of PTPN22 toward pNPP (F,G). Data information: All Experiments were carried three times (A-E) with similar results. In (A-C), Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.
Immunosorbent Assay, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 2rα  (Multi Sciences (Lianke) Biotech Co Ltd)
94
Multi Sciences (Lianke) Biotech Co Ltd il 2rα
A Compared with the PTPN22 WT, PTPN22 ΔP1 can increase remarkably the phosphorylation levels of ZAP-70, Lck and Erk. B, C, D Western blot and qPCR assays were used to test the protein and mRNA expression levels of <t>CD25,</t> CD69 and IL-2, and the results shown that the protein and mRNA expression levels of CD25, CD69 and IL-2 increased significantly in PTPN22 ΔP1 transfected cells. Western blot assays were used to test the protein expression levels of CD25 and CD69 (B-C) or qPCR assays were used to test the mRNA expression levels of CD25, CD69 and IL-2 (D). E, F, G Deletion of P1 domain of PTPN22 will not affect its enzyme activity. pNPP hydrolysis with PTPN22 catalytic domain and PTPN22 ΔP1 (E) or Kinetics parameters for the wild-type and the mutants of PTPN22 toward pNPP (F,G). Data information: All Experiments were carried three times (A-E) with similar results. In (A-C), Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.
Il 2rα, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cd25/il-2sr alpha elisa kit picokine  (Boster Bio)
90
Boster Bio human cd25/il-2sr alpha elisa kit picokine
A Compared with the PTPN22 WT, PTPN22 ΔP1 can increase remarkably the phosphorylation levels of ZAP-70, Lck and Erk. B, C, D Western blot and qPCR assays were used to test the protein and mRNA expression levels of <t>CD25,</t> CD69 and IL-2, and the results shown that the protein and mRNA expression levels of CD25, CD69 and IL-2 increased significantly in PTPN22 ΔP1 transfected cells. Western blot assays were used to test the protein expression levels of CD25 and CD69 (B-C) or qPCR assays were used to test the mRNA expression levels of CD25, CD69 and IL-2 (D). E, F, G Deletion of P1 domain of PTPN22 will not affect its enzyme activity. pNPP hydrolysis with PTPN22 catalytic domain and PTPN22 ΔP1 (E) or Kinetics parameters for the wild-type and the mutants of PTPN22 toward pNPP (F,G). Data information: All Experiments were carried three times (A-E) with similar results. In (A-C), Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.
Human Cd25/Il 2sr Alpha Elisa Kit Picokine, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa systems specific for ox40, ox40l and cd25  (ELISA SYSTEMS Pty Ltd)
90
ELISA SYSTEMS Pty Ltd elisa systems specific for ox40, ox40l and cd25
A Compared with the PTPN22 WT, PTPN22 ΔP1 can increase remarkably the phosphorylation levels of ZAP-70, Lck and Erk. B, C, D Western blot and qPCR assays were used to test the protein and mRNA expression levels of <t>CD25,</t> CD69 and IL-2, and the results shown that the protein and mRNA expression levels of CD25, CD69 and IL-2 increased significantly in PTPN22 ΔP1 transfected cells. Western blot assays were used to test the protein expression levels of CD25 and CD69 (B-C) or qPCR assays were used to test the mRNA expression levels of CD25, CD69 and IL-2 (D). E, F, G Deletion of P1 domain of PTPN22 will not affect its enzyme activity. pNPP hydrolysis with PTPN22 catalytic domain and PTPN22 ΔP1 (E) or Kinetics parameters for the wild-type and the mutants of PTPN22 toward pNPP (F,G). Data information: All Experiments were carried three times (A-E) with similar results. In (A-C), Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.
Elisa Systems Specific For Ox40, Ox40l And Cd25, supplied by ELISA SYSTEMS Pty Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cd25 elisa set 96 well  (OriGene)
N/A
Human CD25 ELISA Set 10 x 96 well
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Image Search Results


A Compared with the PTPN22 WT, PTPN22 ΔP1 can increase remarkably the phosphorylation levels of ZAP-70, Lck and Erk. B, C, D Western blot and qPCR assays were used to test the protein and mRNA expression levels of CD25, CD69 and IL-2, and the results shown that the protein and mRNA expression levels of CD25, CD69 and IL-2 increased significantly in PTPN22 ΔP1 transfected cells. Western blot assays were used to test the protein expression levels of CD25 and CD69 (B-C) or qPCR assays were used to test the mRNA expression levels of CD25, CD69 and IL-2 (D). E, F, G Deletion of P1 domain of PTPN22 will not affect its enzyme activity. pNPP hydrolysis with PTPN22 catalytic domain and PTPN22 ΔP1 (E) or Kinetics parameters for the wild-type and the mutants of PTPN22 toward pNPP (F,G). Data information: All Experiments were carried three times (A-E) with similar results. In (A-C), Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.

Journal: bioRxiv

Article Title: PTPN22 interacts with EB1 to regulate T cell receptor signaling

doi: 10.1101/481507

Figure Lengend Snippet: A Compared with the PTPN22 WT, PTPN22 ΔP1 can increase remarkably the phosphorylation levels of ZAP-70, Lck and Erk. B, C, D Western blot and qPCR assays were used to test the protein and mRNA expression levels of CD25, CD69 and IL-2, and the results shown that the protein and mRNA expression levels of CD25, CD69 and IL-2 increased significantly in PTPN22 ΔP1 transfected cells. Western blot assays were used to test the protein expression levels of CD25 and CD69 (B-C) or qPCR assays were used to test the mRNA expression levels of CD25, CD69 and IL-2 (D). E, F, G Deletion of P1 domain of PTPN22 will not affect its enzyme activity. pNPP hydrolysis with PTPN22 catalytic domain and PTPN22 ΔP1 (E) or Kinetics parameters for the wild-type and the mutants of PTPN22 toward pNPP (F,G). Data information: All Experiments were carried three times (A-E) with similar results. In (A-C), Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: CD25 ELISA was performed using the pre-packaged CD25 ELISA kit (DY2438, R & D system), while CD69 ELISA was performed using the pre-packaged CD69 ELISA kit (TWp022633, www.tw-reagent.com ) following the manufacturer’s protocols.

Techniques: Western Blot, Expressing, Transfection, Activity Assay

A, B Test the protein expression levels of CD25 and CD69 by use of ELISA. ELISA assays were used to test the expression levels of CD25 (A) or Same assays were used to test the expression levels of CD69 (B). C, D Test the mRNA transcription levels of CD25 and CD69 by use of qPCR. qPCR assays were used to test the expression levels of CD25 (C) or Same assays were used to test the expression levels of CD69 (D). Data information: Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.

Journal: bioRxiv

Article Title: PTPN22 interacts with EB1 to regulate T cell receptor signaling

doi: 10.1101/481507

Figure Lengend Snippet: A, B Test the protein expression levels of CD25 and CD69 by use of ELISA. ELISA assays were used to test the expression levels of CD25 (A) or Same assays were used to test the expression levels of CD69 (B). C, D Test the mRNA transcription levels of CD25 and CD69 by use of qPCR. qPCR assays were used to test the expression levels of CD25 (C) or Same assays were used to test the expression levels of CD69 (D). Data information: Significant differences were calculated with t-test and are indicated with *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: CD25 ELISA was performed using the pre-packaged CD25 ELISA kit (DY2438, R & D system), while CD69 ELISA was performed using the pre-packaged CD69 ELISA kit (TWp022633, www.tw-reagent.com ) following the manufacturer’s protocols.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay